Ephys Link
The Electrophysiology Manipulator Link (or Ephys Link for short) is a Python Socket.IO server that allows any Socket.IO-compliant application (such as Pinpoint) to communicate with manipulators used in electrophysiology experiments.
Currently, Ephys Link only supports Sensapex uMp-4 Micromanipulators and New Scale 3-axis manipulators. However, this platform is designed to be extensible to other manipulators and more may be added in the future.
For more information regarding the server’s implementation and how the code is organized, see the package’s development documentation.
For detailed descriptions of the server’s API, see the API reference.
Installation
Prerequisites
Python 3.8+ and pip.
An x86 Windows PC is required to run the server.
For Sensapex devices, the controller unit must be connected via an ethernet cable and powered. A USB-to-ethernet adapter is acceptable. For New Scale manipulators, the controller unit must be connected via USB and be powered by a 6V power supply.
To use the emergency stop feature, ensure an Arduino with the StopSignal sketch is connected to the computer. Follow the instructions on that repo for how to set up the Arduino.
Using a Python virtual environment is encouraged.
Create a virtual environment by running
python -m venv ephys_link
Activate the environment by running
.\ephys_link\scripts\activate
A virtual environment helps to isolate installed packages from other packages on your computer and ensures a clean installation of Ephys Link
NOTE: Ephys Link is an HTTP server without cross-origin support. The server is currently designed to interface with local/desktop instances of Pinpoint. It will not work with the web browser versions of Pinpoint at this time.
Install for use
Run the following command to install the server:
pip install ephys-link
Update the server like any other Python package:
pip install --upgrade ephys-link
Usage
Run the following commands in a terminal to start the server for the desired manipulator platform:
Manipulator Platform |
Command |
---|---|
Sensapex |
|
New Scale |
|
New Scale via Pathfinder HTTP server |
|
There are a couple additional aliases for the Ephys Link executable: ephys_link
and el
.
By default, the server will broadcast with its local IP address on port 8081. Copy this information into Pinpoint to connect.
For example, if the server is running on the same computer that Pinpoint is, use
Server:
localhost
Port:
8081
“New Scale” vs “New Scale via Pathfinder HTTP server”
New Scale manipulators have two methods of connection. The first method is through a direct hardware
connection (-t new_scale
) and the second is via the HTTP server broadcast by the Pathfinder
software (-t new_scale_pathfinder
). The latter method is only available if Pathfinder is running on the same computer
as Ephys Link. New Scale manipulators should be connected to the Pathfinder software first and the HTTP server should be
running.
Note: Currently (July 2023), Ephys Copilot automation and manual control within Pinpoint is not supported when connected to New Scale via Pathfinder HTTP server Pinpoint is not supported. Only positional data can be queried.
Options
All options can be viewed with python -m ephys_link --help
.
Option |
Input |
Default |
Description |
---|---|---|---|
|
Show help message and options |
||
|
|
|
Select manipulator type |
|
Valid HTTP port number |
|
HTTP port to serve on |
|
Valid HTTP port number |
|
HTTP port Pathfinder’s server is running on |
|
Serial port name (i.e. |
|
Emergency stop serial port. Auto searches if no port specified |
|
Enable verbose logging for debugging |
||
|
Version number of server |